The Antibacterial Effectiveness Of Green Tea Leaf Extract (Camellia Sinensis. L) From Pagar Alam Against Streptococcus Mutans

Maria Salsa Bula; Listia Eka Merdekawati; Danica Anastasia; Tyas Hestiningsih

Authors

Maria Salsa Bula Universitas Sriwijaya Author
Listia Eka Merdekawati Universitas Sriwijaya Author
Danica Anastasia Universitas Sriwijaya Author
Tyas Hestiningsih Universitas Sriwijaya Author

Keywords:

green tea, streptococcus mutans, antibacterial

Abstract

Introduction: Dental caries remains a prevalent oral health issue in Indonesia, with Streptococcus mutans identified as the main causative agent. While 0.2% chlorhexidine is effective, its side effects highlight the need for natural alternatives. Green tea (Camellia sinensis. L) contains antibacterial compounds like catechins and flavonoids.

Purpose: To evaluate the antibacterial effectiveness of green tea leaf extract from Pagar Alam against Streptococcus mutans.

Methods: This in vitro study used a post-test only control group design with the disk diffusion method. Samples consisted of green tea extract at concentrations of 5%, 15%, and 25%, and a 0.2% chlorhexidine as control. Inhibition zones were measured with a caliper and analyzed using One Way ANOVA and Post Hoc LSD tests.

Results: The average inhibition zone diameters were 5.900 mm (5%), 7.908 mm (15%), and 10.033 mm (25%). The positive control showed 16.700 mm. Significant differences were observed among all groups (p<0.05). The 25% concentration yielded the highest inhibitory effect compared to the other concentrations. However, green tea leaf extract from Pagar Alam is still not effective as antibacterial compared to CHX 0,2%.

Conclusion: Green tea leaf extract from Pagar Alam can inhibit significantly the growth of Streptococcus mutans, with increasing antibacterial activity at higher concentrations.

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