The Antibacterial Effectiveness Of N-Hexane Garlic Peel Extract Against Staphylococcus aureus

Salsabila Fauza Rahima; Trisnawaty K; Valentino Haksajiwo; Bambang Nuryadi

Authors

Salsabila Fauza Rahima Universitas Sriwijaya Author
Trisnawaty K Universitas Sriwijaya Author
Valentino Haksajiwo Universitas Sriwijaya Author
Bambang Nuryadi Universitas Sriwijaya Author

Keywords:

antibacterial, garlic peel, n-hexane, Staphylococcus aureus

Abstract

Background: Odontogenic infections are a common disease in dental practice that is caused by bacteria S. aureus. One of herbal ingredients that has antibacterial properties is garlic peel which can be extracted with n-hexane. This has not been widely studied and it is still considered waste. Purpose: This study aimed to determine the effectiveness of antibacterial extract of garlic peel against S. aureus. Materials and Methods: This study is an experimental in vitro laboratory study. The test group used an extract of garlic peel with concentrations of 25%, 50%, 75%, and 100% obtained by maceration method with n-hexane, povidone iodine 1% was used as a positive control and aquades as a negative control. The antibacterial potency was tested using disc diffusion method to determine the value of inhibition zone, the dilution method to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The inhibition zone values were then analyzed statistically using Kruskal Wallis and Mann-Whitney tests. Results: This study showed that average inhibition zone diameter for the 25% and 50% concentrations was 0 mm, indicating no antibacterial activity. Meanwhile, the 75% and 100% concentrations exhibited inhibition zones of 0.06 mm and 0.07 mm, respectively, which were classified as weak inhibition and lower than that of povidone iodine 1%. The MIC and MBC values could not be determined in this study. Conclusion: N-hexane garlic peel extract has antibacterial activity against S. aureus at high concentrations (75% and 100%) and the activity is classified as weak.

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