THE EFFECT OF SUMBAWA FOREST HONEY ON INCREASING THE NUMBER OF FIBROBLAST CELLS (IN VIVO STUDY AFTER WISTAR RAT TOOTH EXTRACTION)

Welmi Liaman; Ickman Setoaji Wibowo; Ulfa Yasmin; Galuh Anggraini Adityaningrum; Trisnawaty K

Authors

Welmi Liaman Universitas Sriwijaya Author
Ickman Setoaji Wibowo Universitas Sriwijaya Author
Ulfa Yasmin Universitas Sriwijaya Author
Galuh Anggraini Adityaningrum Universitas Sriwijaya Author
Trisnawaty K Universitas Sriwijaya Author

Keywords:

Sumbawa forest honey, tooth extraction, Fibroblast, wound healing

Abstract

Background: Fibroblasts play an important role in the process of wound healing in the proliferation phase. Honey contains flavonoids, phenolic acids, vitamins and enzymes that play a role in increasing fibroblasts. The aim of this research was to determine the effect of Sumbawa forest honey on increasing the number of fibroblast cells after Wistar rat tooth extraction on day 7. Method: This is a laboratory experimental study with Post Test Only with Control Group design. In this study used 24 male Wistar rats, divided into 4 groups, the negative control (aquadest), the 50% Sumbawa forest honey, the 100% Sumbawa forest honey, and the positive control (hyaluronic acid) were applied topically twice a day for 7 days in dental sockets after tooth extraction in Wistar rats. The number of fibroblast was counted by histopathology preparations under Olympus CX31 microscope and ImageJ software and analyzed using Independent sample t-test and Post Hoc Least Significant Difference (LSD). Results: The result of this study showed the group with 100% consentration of Sumbawa forest honey had significantly higher number of fibroblas cells compared to the group with 50% consentration of Sumbawa forest honey and the negative control (p<0.05). Conclusion: Sumbawa forest honey concentrations of 50% and 100% have an effect in increasing the number of fibroblast cells after tooth extraction of wistar rats and 100% have an effect in increasing the number of fibroblast cells after tooth extraction of wistar rats and the highest number of fibroblast cells was found in the group with concentration of 100% Sumbawa forest honey.

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