EFFECTIVENESS OF ROBUSTA SEMENDO COFFEE EXTRACT IN THE TREATMENT OF CHRONIC PERIODONTITIS ON REDUCING THE COUNT OF PMN CELLS IN WISTAR RATS
Keywords:
Semendo Robusta coffee, chronic periodontitis, PMN cells, anti-inflammatory, wistar ratsAbstract
Background: Periodontitis is an oral disease with a very high prevalence in Indonesia. Periodontitis can cause tooth loss. Semendo robusta coffee extract contains active compounds as anti-inflammatory and antibacterial which can reduce the number of PMN cells in chronic periodontitis treatment. Objective: This study aimed to determine the effectiveness of Semendo Robusta coffee bean extract in reducing PMN cell counts in the treatment of chronic periodontitis in Wistar rats. Methods: his study used a post test only control group design. A total of 21 male Wistar rats were divided into 3 treatment groups. Making periodontitis rats is done by inducing Porphyromonas gingivalis bacteria into the gingival sulcus of rats for 14 days. Periodontitis rats were applied gel according to the treatment group twice a day for 14 days. Semendo robusta coffee extract gel applied to periodontitis rats with various concentrations of 40%, 50%, and placebo gel (negative control). All samples were euthanised on the 15th day after gel application and histological sections were made. The number of PMN cells was counted and statistically analysed. Results: Semendo robusta coffee extract gel group with 50% concentration was the group with the lowest PMN cell value. Statistically, the 40% Semendo coffee extract gel group had a significant difference (p<0.05) compared to the placebo gel group. Conclusion: Semendo robusta coffee bean extract gel with 50% concentration significantly decreased the number of PMN cells against chronic periodontitis treatment in Wistar rats compared to 40% semendo robusta coffee extract gel and placebo gel.
References
Kementrian kesehatan Republik Indonesia. Badan penelitian dan pengembangan kesehatan. 2018.
Martínez GM, Hernandez LE. Periodontal inflammation and systemic diseases: an overview. Front Physiol. 2021;12(1); p.1–26.
Andriani I, Chairunnisa F. Case report periodontitis kronis dan penatalaksaan kasus dengan kuretase. Insisiva Dental Journal Majalah Kedokteran Gigi Insisiva. 2019;8(1);p.25–30.
Prasetya RC, Praharani D, Fatimatuzzahro N, Ermawati T, Tsalats. The effect of brewed robusta coffee (Coffea canephora) on macrophage and lymphocyte cells in rat model of chronic periodontic. Padjadjaran Journal Of Dental Researcher and Students. 2021;5(1);p.18.
Nuryadi B, Zakyah A, Vidyasari AS. Antibacterial effect of semendo coffee beans (Coffea canephora) extract against Streptococcus sanguinis in vitro growth. Denta Jurnal Kedokteran Gigi. 2021;15(1);p.1–8.
Sulistiawati S, Awalia H, Yasmin U, Dwi RS, Namira A. The effect of semendo coffee on the number of fibroblast cells in gingiva wound of rats. B-Dent Jurnal Kedokteran Gigi Universitas Baiturrahmah. 2022;9(1);p.19– 25.
Rahardjo P. Panduan berkebun kopi. Jakarta, PA: Penebar Swadaya Grup;2017;p.5-7.
Saputro R, Idris M, Sari L, Chairunisa E. Perkembangan kopi semende kurun waktu 1919-2019 sebuah tinjauan studi. Jurnal Sejarah dan Pembelajaran Sejarah. 2020;6(1);p.18-24.
Anwari RH. Dampak konsumsi kopi pada penurunan kadar glukosa darah penderita diabetes mellitus tipe 2. Jurnal Penelitian Perawat Profesional. 2021;3(3);p.531–40.
Husodo B. Konsumsi kopi untuk mencegah penyakit alzheimer. Jurnal Ilmiah Kesehatan Sandi Husada. 2020;12(2);p.996–1002.
Nieber K. The impact of coffee on health author pharmacokinetics and mode of action bioactive components in coffee. Planta Med. 2017;83(1);p.1256–63.
Dewajanti AM. Peranan asam klorogenat tanaman kopi terhadap penurunan kadar asam urat dan beban oksidatif. Jurnal Kedokteran Meditek. 2019;25(1);p.46–51.
Ermawati T, Prasetya RC, Fatimatuzzahro, Ganadya NIA. Efek gel ekstrak biji kopi robusta (Coffea canephora) terhadap jumlah sel makrofag dan limfosit jaringan gingiva tikus periodontitis. Insisiva Dental Journal Makalah Kedokteran Gigi Insisiva. 2021;9(2);p.46-8.
Dianastri RN, Astuti P, Prasetya RC. Daya hambat ekstrak biji kopi robusta (Coffea canephora) terhadap bakteri Porphyromonas gingivalis (in vitro). Stomatognatic (Jkg Unej). 2021;18(2);p.69–73.
Newman MG, Takei HH, Klokkevold PR, Carranza FA. Carranza’s clinical periodontology clinical evaluation of the implant patient. 13th Ed. Philadelphia, PA: Elsevier;2018;P 635–48.
Newman MG, Essex G, Laughter L, Elangovan S. Newman and carranza clinical periodontology for the dental hygienist. Philadelphia, PA: Elsevier;2020;p.318-29.
Rathee M, Jain P. Gingivitis [Internet]. San Francisco: Statpearls Publishing; 2020 [updated 2023 may 27; cited 2020 june 3]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK557422/ .
Reddi S. Essentials of clinical periodontology and periodontic. 3th Ed. New Delhi, PA: Jaypee Brother Medical Publishers;2013;p.82
Duzgunes N. Medical microbiology and immunology. United States, PA: Quintessence Publishing;2019;p.356.
Palmer R, Floyd P. Periodontology. 4th Ed. London, PA: Springer;2021;p.31- 44.
Wijaksana EKI. Perio dx: periodontal sehat, gingivitis dan periodontitis. Jawa Timur, PA: Airlangga University Press;2020;p.63-83
Xu W, Zhou W, Wang H, Liang S. Roles of Porphyromonas gingivalis and its virulence factors in periodontitis. Advances in Protein Chemistry and Structural Biology. 2020;120(4);p.45–84.
Bostanci N, Belibasakis GN. Pathogenesis of periodontal diseases: biological concepts for clinicians. London, PA: Springer;2018;p.73-89.
Zaric SS, Lappin MJ, Fulton CR et al. Sialylation of Porphyromonas gingivalis lps and its effect on bacterial host interactions. Innate Immununity. 2017;23(3);p.319–26.
Putri CF, Bachtiar EW. Porphyromonas gingivalis dan patogenesis disfungsi kognitif: analisis peran sitokin neuroinflamasi. Cakradonya Dental Journal. 2020;12(1):15–23.
Rahmadani N, Aris Wahyukundari M, Harmono H, Kunci K. Effectivity of robusta coffe bean (Coffea canephora) extract gel against fibroblast enhancement on post gingivectomy wound healing. Stomatognatic Jurnal Kedokteran Gigi Unej. 2022;9(1);p.13–8.
Fuadiyah D, Ratnawaty R, Soebijakto BA. Pengaruh lama paparan lps (lipopolisakarida) Phorpyromonas gingivalis sebagai induktor periodontitis terhadap kadar ldl dan hdl tikus wistar (Rattus norvegicus). E-Prodenta Journal of Dentistry. 2017;1(2);p.54-67.
Septiwidyati TR, Bachtiar EW. The role of Porphyromonas gingivalis
proteinases in periodontitis. Dentika Dental Journal. 2020;51(2);p.149–69.
Mysak J, Podzimek S, Sommerova P, et al. Porphyromonas gingivalis major periodontopathic pathogen overview. Journal of Immunology Research. 2014;1(1);p.1-8.
Farhaty N. Tinjauan kimia dan aspek farmakologi senyawa asam klorogenat pada biji kopi: review. Farmaka. 2012;14(3);p.214–27.
Prastowo B, Karmawati E, Indrawanto C, Munarso SJ. Budidaya dan pasca panen kopi. Jakarta, PA: Eska Media;2013;p.4-10.
Chandra D, Ismono H, Kasymir E. Prospek perdagangan kopi robusta indonesia di pasar internasional. Jurnal Ilmu Agribisnis. 2013;1(1);p.110–5.
Abduh YM. Dari institut teknologi bandung untuk Indonesia biorefinery kopi. Bandung, PA: Institut Teknologi Bandung Press;2018;p.3.
Tetti M. Ekstraksi, pemisahan senyawa, dan identifikasi senyawa aktif. Jurnal Kesehatan. 2014;7(2);p.2.
Tungadi R. Teknologi nano sediaan liquida dan semisolida. Jakarta, PA: CV.Sagung Seto;2020;p.263
Agustiani FRT, Sjahid LR, Nursal FK. Kajian literatur: peranan berbagai jenis polimer sebagai gelling agent terhadap sifat fisik sediaan gel. Majalah Farmasetika. 2022;7(4);p.270.
Zakir Z, Hassan M. Caffeine (1,3,7-trimethylxanthine): the good and the bad: a review. Journal of Public Health and Biological Sciences. 2013;2(4);p.313.
Wenas DM, Aliya LS, Janah NU. Aktivitas antiinflamasi ekstrak etanol daun kopi arabika (Coffea arabica L.) pada edema tikus. Buletin Penelitian Tanaman Rempah dan Obat. 2020;31(2);p.75.
Ermawati T. Potensi gel ekstrak biji kopi robusta (Coffea robusta). Fakultas Kedokteran Gigi Universitas Jember. 2015;1(1);P.3–12.
Fitria L. Profil hematologi tikus (Rattus norvegicus berkenhout, 1769) galur wistar jantan dan betina umur 4, 6, dan 8 minggu. Biogenesis: Jurnal Ilmiah Biologi. 2014;2(2);p. 94 – 100.
Fitria L, Lukitowati F, Kristiawati D. Nilai rujukan untuk evaluasi fungsi hati dan ginjal pada tikus (Rattus norvegicus berkenhout, 1769) galur wistar. Jurnal Pendidikan Matematika dan IPA. 2019;10(2);p.81.
Kartika AA, Siregar, Fuah A. Strategi pengembangan usaha ternak tikus (Rattus norvegicus) dan mencit (Mus musculus) di fakultas peternakan ipb. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan. 2013;1(3);p.147–54.
Frianto F. Evaluasi faktor yang mempengaruhi jumlah perkawinan tikus putih (Rattus norvegicus) secara kualitatif. Jurnal Mahasiswa Farmasi Fakultas Kedokteran Untan. 2015;3(1);p.2.
Alexandru I. Experimental use of animals in research spa. Journal Balneo Research. 2018;2(1);p.25–9.
Budhy T, Arundina I, Surboyo M, Halimah A. The effects of rice husk liquid smoke in Porphyromonas gingivalis-induced periodontitis. European Journal of Dentistry. 2021;15(4);p.653–9.
Putri RR, Hakim RF, Rezeki S. Pengaruh ekstrak daun tapak dara (Catharanthus roseus) terhadap jumlah fibroblas pada proses penyembuhan luka di mukosa oral. Caninus Denstistry. 2017;2(1);p.20–30.
Paulita M, Bhakti Purnamasari C, Yani S, Dihin Utami N. Uji efektivitas antiinflamasi ekstrak etanol daun sembung (Blumea balsamifera) terhadap gingiva tikus wistar putih pasca induksi Porphyromonas gingivalis. Mulawarman Dental Jurnal. 2021;1(1):1–9.
Wulandari A, Arina YMD, Pujiastuti P. Daya hambat pasta gigi yang mengandungekstrak biji kopi robusta (Coffea canephora) terhadap pertumbuhan bakteri Porphyromonas gingivalis. Jurnal Penelitian Kesehatan Suara Forikes. 2023;4(1):31–6.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
CC Attribution-NonCommercial-ShareAlike 4.0
